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Platycodonis Radix is the dry root of Platycodon grandiflorum of Campanulaceae, which has a variety of pharmacological effects and is a commonly used bulk Chinese medicine. In this study, the chloroplast genome sequences of six P. grandiflorum from different producing areas has been sequenced with Illumina HiSeq X Ten platform. The specific DNA barcodes were screened, and the germplasm resources and genetic diversity were analyzed according to the specific barcodes. The total length of the chloroplast genome of 6 P. grandiflorum samples was 172 260-172 275 bp, and all chloroplast genomes showed a typical circular tetrad structure and encoded 141 genes. The comparative genomics analysis and results of amplification efficiency demonstrated that trnG-UCC and ndhG_ndhF were the potential specific DNA barcodes for identification the germplasm resources of P. grandiflorum. A total of 305 P. grandiflorum samples were collected from 15 production areas in 9 provinces, for which the fragments of trnG-UCC and ndhG_ndhF were amplificated and the sequences were analyzed. The results showed that trnG-UCC and ndhG_ndhF have 5 and 11 mutation sites, respectively, and 5 and 7 haplotypes were identified, respectively. The combined analysis of the two sequences formed 13 haplotypes (named Hap1-Hap13), and Hap4 is the main genotype, followed by Hap1. The unique haplotypes possessed by the three producing areas can be used as DNA molecular tags in this area to distinguish from the germplasm resources of P. grandiflorum from other areas. The haplotype diversity, nucleotide diversity and genetic distance were 0.94, 4.79×10-3 and 0.000 0-0.020 3, respectively, suggesting that the genetic diversity was abundant and intraspecific kinship was relatively close. This study laid a foundation for the identification of P. grandiflorum, the protection and utilization of germplasm resources, and molecular breeding.
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Objectives@#To determine the efficacy of low-dose heparin in preventing central catheter occlusion and its safety among neonates.@*Materials and Methods@#A randomized controlled trial was conducted among 42 neonates requiring peripherally inserted central catheter (PICC) lines. The neonates were divided into two groups: low dose heparin (0.5 units/kg/hr =0.2 units/ml) and control group (0.5 units/ml). The efficacy outcomes were duration of catheter patency, completion of catheter use, and the presence of catheter occlusion or thrombosis. The safety outcomes include heparin complications.@*Results@#The study participants had a mean age of 17 days old at 35 weeks gestational age and mean weight of 1.97 kg. The participants given low dose heparin were 36% more likely to complete the use of central line and 12% less likely to develop catheter occlusion. Analyses showed non-statistically significant risk ratio of active bleeding, thrombocytopenia, and deranged prothrombin time in the low dose heparin group.@*Conclusion@#The use of low dose heparin (0.5 units/kg/hr = 0.2 units/ml) appears as effective as the control dose in completion of catheter use and prevention of catheter occlusion. There was also no significant difference in the adverse effects. Low dose heparin can be used as continuous infusion for preventing central line occlusion; however, it has no advantage in lowering the risk of complications.
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HemorrhageABSTRACT
Three children with Skene duct cyst were presented in this article. By reviewing literature, in pediatric population, Skene duct cycts mostly occur in newborns and conservative therapy is the first choice in this group.In contrast, it is extremely rare between the ages of 1 and 12, and surgical excised is the preferred therapy because of having a similar pathogenesis to adults.
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Objective:To investigate clinicopathological characteristics of misdiagnosed pediatric extraskeletal Ewing′s sarcoma in children, its diagnosis and differential diagnosis, and the reasons of misdiagnosis by pathologists.Methods:The clinical data of 34 cases of pediatric extraskeletal Ewing′s sarcoma diagnosed by the Department of Pathology, Xi′an Children′ Hospital and Xijing Hospital Fourth Military Medical University from January 2011 to July 2019 were retrospectively analyzed.Hematoxylin-eosin staining, immunohistochemical staining and EWSR1 fusion gene detection by fluorescence in situ hybridization (FISH) were performed on 6 primarily misdiagnosed cases.The clinical features, histological morphology, immunophenotype and molecular genetics of these 6 patients were studied. Results:The median age of 6 cases was 7 years old.There were 5 cases with slowly-growing, painless and localized masses.Four masses were located in the superficial subcutaneous fascia, and 1 was a rapidly growing soft tissue mass.Tumor cells were lobular and nest-like under low power microscopy.At high power microscopy, 2 cases were composed of small blue round cells, and 4 cases had morphological changes, including an increase in cell volume and enriched cytoplasm.Among the 4 cases with tumor morphological changes, tumor cells of 1 case had fine and smooth chromatin, tumor cells of 1 case had clear nuclei and prominent nucleoli, tumor cells of 1 case were composed of epithelioid cells, and tumor cells of 1 case were mixed spindle and epithelioid cells in large round and oval cells.The positive rates of CD 99, friend leukaemia integration-1(FLI1) and ERG were 5/6 cases, 5/6 cases and 2/6 cases, respectively.Rearrangements of EWSR1 gene were detected in all 6 cases. Conclusions:Biological characteristics including young onset age, superficial position and primary benign tumor, histological diversity and atypical immunohistochemical markers are 3 important factors contributing the misdiagnosis of extraskeletal Ewing′s sarcoma in children.The diagnosis should be made by combining the clinical features, morphology, immunohistochemical staining and EWSR1 gene detection.
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Objective To understand the thyroid stimulating hormone (TSH) level of neonates and iodine nutritional status of pregnant women and children in Guizhou Province.Metheds From 2016 to 2017,a city (district,county) in government location in Guizhou Province was selected as the urban survey site,and a county (city,district) whose economy was relatively backward and farther from the city (prefecture) in government location was selected as the rural survey site.In each survey site,300 salt samples were collected for determination of salt iodine;300 blood samples in the heel of neonates after birth 72 hours were taken for measurement of TSH.The Wodd Health Organization,United Nations International Children's Emergency Fund,and the International Committee for the Control of Iodine Deficiency (WHO/UNICEF/ICCIDD) recommend that when the proportion of neonates (TSH levels ＞ 5 mU/L) is ＜ 3％,the area is non-iodine-deficient.In other words,the 97th percentile (P97) value should be ≤5 mU/L.The normal reference value of the kit is ≤9 mU/L.And urinary iodine of 150 children aged 8-10 and 150 pregnant women was determined.Results A total of 5 400 edible salt samples were tested,the median of salt iodine was 27.9 mg/kg,and the consumption rate of qualified iodized salt was 94.89％ (5 124/5 400);5 400 neonatal heel blood samples were collected,the median of TSH was 2.62 mU/L,TSH P97 was 6.91 mU/L,and samples proportion with TSH ＞ 5 mU/L was 10.67％ (576/5 400),and ＞ 9 mU/L (the cut-off value of kit) was 1.00％ (54/5 400).Among 3 588 cases of children aged 8 to 10 years,the median of urinary iodine was 212.3 μg/L,those with urinary iodine ＜ 50 μg/L was 2.81％ (101/3 588),and ＜ 100 μg/L was 12.76％ (458/3 588).Among the 2 695 pregnant women,the median of urinary iodine was 163.1 μg/L,and 44.04％ (1 187/2 695) were ＜ 150 μg/L.Conclusions The quality of iodized salt is stable in Guizhou,the iodine nutrition status of children and pregnant women are good.The neonatal TSH and nutritional status of pregnant women should be monitored in the future.
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<p><b>OBJECTIVE</b>To analyze the epidemiological features of childhood cancer in China, and to provide some clues and basis for related academic research, the formulation of prevention and control strategy, and the construction of prevention and control system of childhood cancer.</p><p><b>METHODS</b>The data of childhood cancer in children aged 0-14 years which were published in Chinese Cancer Registry Annual Report in 2009-2012 and from GLOBOCAN2012 database were collected. A descriptive statistical analysis was done to determine the distributions of incidence and mortality by time, area, age, and sex.</p><p><b>RESULTS</b>In China, the incidence of childhood cancer showed a slight fluctuation with time, while the mortality rate remained stable. The incidence of childhood cancer in China was lower than the world average, and it was much lower than in the United States and Japan. However, the mortality of childhood cancer in China was higher than that in the United States and Japan. The incidence of childhood cancer in Chinese urban areas was about 2 times that in Chinese rural areas, while the mortality in the urban and rural areas was similar. The incidence and the mortality of childhood cancer both declined with increasing age. The incidence and the mortality of childhood cancer in boys were higher than those in girls.</p><p><b>CONCLUSIONS</b>The incidence and mortality of childhood cancer in China show distribution characteristics by time, area, age, and sex, which can help to make clear the future direction of childhood cancer research and provide some ideas for the comprehensive prevention and control.</p>
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Adolescent , Child , Child, Preschool , Female , Humans , Infant , Infant, Newborn , Male , China , Epidemiology , Neoplasms , Epidemiology , MortalityABSTRACT
Objective To describe MRI findings of 12 cases of non hemorrhagic contusion of corpus callosum. Methods The MR images of 12 patients were reviewed retrospectively. 11 cases were victims of vehicle accident and 1 patient was injured by falling. All patients were examined with precontrast CT and MR imaging within 3 days (8 cases) and 6 to 30 days (4 case). Results The lesions were located at splenium (3 cases) and mainly at body (9 cases). There was no abnormal signs of corpus callosum on CT images. MR study in all 12 cases demonstrated isointense or slight hypointense signal compared with gray matter on T 1WI and obvious hyperintense signal lesions on T 2WI on corpus callosum. There was no hemorrhagic signal or density on MR and CT images at corpus callosum. These contusion lesions were demonstrated nicely on SE or FSE T 2WI images. On FLAIR sequence, these lesions were revealed more clearly for the signal of CSF was suppressed. MR follow up examination in one case four months later, the contusion lesion became malacia with the signal similar to that of CSF. Several other multiple contusion and hemorrhage lesions were detected in ten cases. Conclusion The non hemorrhagic contusion of corpus callosum is a relatively rare species. These lesions can′t be found on CT images, while MRI is the best tool for detecting them. SE or FSE T 2WI is the main sequence for demonstrating. These lesions could be seen more clearly on FLAIR sequence. The isointense or slight hypointense T 1WI signal and hyperintense T 2WI signal of corpus callosum after head injury is the typical MR imaging signs.